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Fluorochem Ltd fluorochem hd2
Fluorochem Hd2, supplied by Fluorochem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorochem+hd2/fluorochem+hd2/pmc05966817-137-11-10
Average 90 stars, based on 1 article reviews
fluorochem hd2 - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Removal of interscapular brown adipose tissue increases aortic stiffness despite normal systemic glucose metabolism in mice
Article Snippet: Adipose tissue blots were corrected to total protein stain on the membrane (1% amido black; Sigma-Aldrich) or blot standard.

Control:

Article Title: Loss of UCP1 exacerbates Western diet-induced glycemic dysregulation independent of changes in body weight in female mice
Article Snippet: UCP-1 (no. U6382, 1:1,000) antibody was purchased from Sigma-Aldrich. .. Intensity of individual protein bands were quantified using FluoroChem HD2 (AlphaView, version 3.4.0.0) and were expressed as a ratio to control band β-tubulin. .. Intensity of individual protein bands were quantified using FluoroChem HD2 (AlphaView, version 3.4.0.0) and were expressed as ratio to control band β-tubulin.

Article Title: Ablation of eNOS does not promote adipose tissue inflammation.
Article Snippet: Nitrotyrosine (no. 5411; 1:500) antibody was purchased from Millipore (Billerica, MA). .. Intensity of individual protein bands was quantified using FluoroChem HD2 (AlphaView, version 3.4.0.0) and were expressed as a ratio to control band GAPDH. ..

Article Title: Aerobic exercise training improves insulin-induced vasorelaxation in a vessel-specific manner in rats with insulin-treated experimental diabetes.
Article Snippet: Membranes were probed with the following primary antibodies: eNOS (610296; 1:500) and phospho-specific eNOS at Ser1177 (61239; 1:250) from BD Biosciences; Akt (4691; 1:500), phospho-specific Akt at Ser473 (4061; 1:250), p44/42-MAPK (4695; 1:500), phospho-specific p44/42-MAPK at Thr202/Tyr/204 (4370; 1:250) and GAPDH (5174, 1:1000) from Cell Signalling; and ET-1 (117757; 1:2000) from Abcam. .. Intensity of individual protein bands was quantified using FluoroChem HD2 (AlphaView, version 3.4.0.0) and expressed as a ratio to control band of GAPDH. ..

Article Title: Loss of UCP1 exacerbates Western diet-induced glycemic dysregulation independent of changes in body weight in female mice.
Article Snippet: Intensity of individual protein bands were quantified using FluoroChem HD2 (AlphaView, version 3.4.0.0) and were expressed as a ratio to control band -tubulin. .. Intensity of individual protein bands were quantified using FluoroChem HD2 (AlphaView, version 3.4.0.0) and were expressed as ratio to control band -tubulin. ..

Article Title: Estrogen receptor-α signaling maintains immunometabolic function in males and is obligatory for exercise-induced amelioration of nonalcoholic fatty liver
Article Snippet: Peroxisome proiferator-activated receptor-γ coactivator 1α (PGC1α; no. 516557, 1:1,000) and CD36 (sc-7309, 1:1,000) primary antibodies were purchased from Millipore, Sigma, and Santa Cruz Biotechnology, respectively. .. Intensities of individual protein bands were quantified using FluoroChem HD2 (AlphaView, v. 3.4.0.0) and expressed as ratio to control band β-tubulin or total protein stain (1% amido-black; Millipore, Sigma). ..

Article Title: Microvascular insulin resistance in skeletal muscle and brain occurs early in the development of juvenile obesity in pigs.
Article Snippet: Omental adipose samples were also probed for -tubulin (no. 2146, 1:1,000; Cell Signaling). .. Intensity of individual protein bands were quantified via densitometry using FluoroChem HD2 (AlphaView, version 3.4.0.0) and were expressed as the ratio of phosphorylated to total Akt, normalized to gel and relative to the control group. ..

Article Title: Loss of Nlrp3 Does Not Protect Mice from Western Diet-Induced Adipose Tissue Inflammation and Glucose Intolerance
Article Snippet: Protein samples (10 μg/lane) were separated by SDS-PAGE, transferred to polyvinylidene difluoride membranes, and probed with Mac-2 (#12733, 1:1000, Cell Signaling) and pro-caspase-1 and p10 caspase-1 (SC-514, 1:500, Santa Cruz). .. Intensity of individual protein bands were quantified using FluoroChem HD2 (AlphaView, version 3.4.0.0), and expressed as ratio to control band GAPDH (#5174, 1:1000, Cell Signaling) or β-tubulin (#2146, 1:1000, Cell Signaling). ..

Staining:

Article Title: Estrogen receptor-α signaling maintains immunometabolic function in males and is obligatory for exercise-induced amelioration of nonalcoholic fatty liver
Article Snippet: Peroxisome proiferator-activated receptor-γ coactivator 1α (PGC1α; no. 516557, 1:1,000) and CD36 (sc-7309, 1:1,000) primary antibodies were purchased from Millipore, Sigma, and Santa Cruz Biotechnology, respectively. .. Intensities of individual protein bands were quantified using FluoroChem HD2 (AlphaView, v. 3.4.0.0) and expressed as ratio to control band β-tubulin or total protein stain (1% amido-black; Millipore, Sigma). ..



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